Rr320^tm4Mkg
Targeted Allele Detail

Summary

• Symbol: Rr320^tm4Mkg
• Name: regulatory region 320; targeted mutation 4, Mark Groudine
• MGI ID: MGI:3621027
• Synonyms: delta1neo, Hbb-ar^tm4Mkg
• Gene: Rr320 Location: Chr7:103501973-103526752 bp, + strand Genetic Position: Chr7, Syntenic
• Alliance: Rr320^tm4Mkg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106655
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to remove 5'HS1 encoded by nucleotides -4675 to -6999 relative to the Ey cap site. The removed sequence includes the core binding site, the region to which the HS maps and all regions of homology with human 5'HS1. A loxP site flanked neomycin resistance gene cassette was included in the vector. The presence of the marker had a substantial effect on expression of beta-like globin genes at all stages. (J:106655)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr320 Mutation: 0 strains or lines available

References

• Original: J:106655 Bender MA, et al., Targeted deletion of 5'HS1 and 5'HS4 of the beta-globin locus control region reveals additive activity of the DNaseI hypersensitive sites. Blood. 2001 Oct 1;98(7):2022-7
• All: 1 reference(s)