Prkdc^{Tg(INS-PDE2)5-1Lmb}
Transgenic Allele Detail

Summary

• Symbol: Prkdc^{Tg(INS-PDE2)5-1Lmb}
• Name: protein kinase, DNA activated, catalytic polypeptide; transgene insertion 5-1, Laboratory of Molecular Biology
• MGI ID: MGI:3620758
• Synonyms: Prkdc^{TgN(InsSRA5)slipLmb}, slip, Sra5-1
• Gene: Prkdc Location: Chr16:15455730-15660099 bp, + strand Genetic Position: Chr16, 10.09 cM, cytoband B1
• Alliance: Prkdc^{Tg(INS-PDE2)5-1Lmb} page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Hypomorph)
• Mutation: Insertion
• Mutation details: A transgene containing an expression construct for yeast cAMP phosphodiesterase (SRA5) under the control of the human insulin promoter randomly inserted into the Prkdc gene, disrupting the normal function of this gene. Expression of Prkdc was shown to be abolished by RT-PCR analysis on RNA derived from homozygous spleen and thymus. Expression of the transgene was negligible. Complementation analysis with Prkdc^scid mice confirmed that the insertion mutation is an allele of the Prkdc gene. (J:44390)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkdc Mutation: 408 strains or lines available

References

• Original: J:44390 Jhappan C, et al., DNA-PKcs: a T-cell tumour suppressor encoded at the mouse scid locus. Nat Genet. 1997 Dec;17(4):483-6
• All: 4 reference(s)