Gja1^tm6(Gjb3)Kwi
Targeted Allele Detail

Summary

• Symbol: Gja1^tm6(Gjb3)Kwi
• Name: gap junction protein, alpha 1; targeted mutation 6, Klaus Willecke
• MGI ID: MGI:3620583
• Synonyms: Cx43^flox, floxCx43KI31neo
• Gene: Gja1 Location: Chr10:56253297-56266519 bp, + strand Genetic Position: Chr10, 28.64 cM
• Alliance: Gja1^tm6(Gjb3)Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:106509
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites flank the coding region of Gja1 and a frt-flanked neomycin resistance cassette that immediately follows the termination codon. The coding region and 88 bp of downstream sequence from Gja3 have been inserted between the Gja1 termination codon and its downstream DNA so that Cre recombinase mediated recombination will delete the Gja1 coding sequence and place that of Gja3 under control of Gja1 regulatory sequences. The GJA1 protein level is slightly elevated in heart, but is not altered in brain, of mice heterozygous or homozygous for the present allele. As in wild-type mice, the GJA3 protein is not expressed in brain or heart. (J:106509)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gja1 Mutation: 59 strains or lines available

References

• Original: J:106509 Zheng-Fischhofer Q, et al., Connexin31 cannot functionally replace connexin43 during cardiac morphogenesis in mice. J Cell Sci. 2006 Feb 15;119(Pt 4):693-701
• All: 1 reference(s)