Vezt^tm1Smc
Targeted Allele Detail

Summary

• Symbol: Vezt^tm1Smc
• Name: vezatin, adherens junctions transmembrane protein; targeted mutation 1, Marie C Simmler
• MGI ID: MGI:3618233
• Synonyms: Vezatin^flox, Vezt^fl
• Gene: Vezt Location: Chr10:93797384-93871661 bp, - strand Genetic Position: Chr10, 48.72 cM
• Alliance: Vezt^tm1Smc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121686
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site resides immediately upstream of exon 5, which is followed by a loxP-flanked Pgk1-neo cassette. Crossing mice homozygous for this allele to mice expressing Cre recombinase under control of a selected promoter results in developmental-stage or tissue-specific deletion of exon 5, which encodes the transmembrane domain shared by all three predicted isoforms of the protein. Reverse transcription- (RT-) PCR demonstrated expression of the expected 8-kb transcript in ES cells and in 8-cell- and blastocyst-stage embryos homozygous for this mutation. (J:121686)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vezt Mutation: 36 strains or lines available

References

• Original: J:121686 Hyenne V, et al., Conditional knock-out reveals that zygotic vezatin-null mouse embryos die at implantation. Mech Dev. 2007 Jul;124(6):449-62
• All: 3 reference(s)