Pofut1^tm2Pst
Targeted Allele Detail

Summary

• Symbol: Pofut1^tm2Pst
• Name: protein O-fucosyltransferase 1; targeted mutation 2, Pamela Stanley
• MGI ID: MGI:3617318
• Synonyms: Pofut1^F
• Gene: Pofut1 Location: Chr2:153083453-153112167 bp, + strand Genetic Position: Chr2, 75.41 cM, cytoband H2
• Alliance: Pofut1^tm2Pst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102175
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 2. (J:83299, J:102175)

Expression

In Mice Carrying this Mutation:	1 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pofut1 Mutation: 22 strains or lines available

Notes

In J:185823, deletion of the floxed Pofut1^tm2Pst alleles by Tg(Sycp1-cre)4Min occurred with only ~5% efficiency, whereas deletion of the floxed Pofut1^tm2Pst alleles by Tg(Prm-cre)58Og occurred with ~50% efficiency. Notably, deletion of the floxed Pofut1^tm2Pst alleles by Tg(Stra8-icre)1Reb was significantly more (~87%) efficient. Finally, deletion of the floxed Pofut1^tm2Pst alleles by Plekha5^{Tg(AMH-cre)1Flor} was significantly less efficient in Sertoli cells cultured from 3-wk-old than in Sertoli cells from 6-month-old homozygous males.

References

• Original: J:102175 Shi S, et al., Canonical Notch signaling is dispensable for early cell fate specifications in mammals. Mol Cell Biol. 2005 Nov;25(21):9503-8
• All: 21 reference(s)