Tcrg^tm1Scar
Targeted Allele Detail

Summary

• Symbol: Tcrg^tm1Scar
• Name: T cell receptor gamma chain; targeted mutation 1, Simon R Carding
• MGI ID: MGI:3615270
• Synonyms: TCR-Vgamma1^-, Vgamma1^-
• Gene: Tcrg Location: Chr13:19362212-19540646 bp, + strand Genetic Position: Chr13, cytoband A2-A3

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105477
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed to replace the T-cell receptor gamma variable 1 gene as well as the joining region and exons 1 and 2 of the T-cell receptor gamma constant 4 gene with a neomycin resistance cassette. Removal of this region would prevent any rearrangement and the possibility of pairing other variable gamma genes with an otherwise intact constant 4 gene. Absence of Tcrg-V1 mRNA in RT-PCR of mutant spleen and thymus confirmed successful ablation. (J:105477)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrg Mutation: 1 strain or line available

References

• Original: J:105477 Andrew EM, et al., Delineation of the function of a major gamma delta T cell subset during infection. J Immunol. 2005 Aug 1;175(3):1741-50
• All: 9 reference(s)