Dntt^tm1Tok
Targeted Allele Detail

Summary

• Symbol: Dntt^tm1Tok
• Name: deoxynucleotidyltransferase, terminal; targeted mutation 1, Toshihisa Komori
• MGI ID: MGI:3613594
• Synonyms: lckTdT⁺
• Gene: Dntt Location: Chr19:41017714-41047964 bp, + strand Genetic Position: Chr19, 34.32 cM
• Alliance: Dntt^tm1Tok page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61801
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced the endogenous promoter with the lck promoter, which is active in developing thymus. Northern blot analysis using a full length cDNA probe detected Dntt transcript in thymus of homozygous mutant E14.5-E18.5 embryos and in adults, as well as in homozygous mutant adult spleen, bone marrow, lymph node and lung, but not in mutant adult kidney, brain or liver. The Dntt expression pattern observed in the mutant mice was identical to that of the Lck gene in normal mice. (J:61801, J:75921)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dntt Mutation: 34 strains or lines available

References

• Original: J:61801 Aono A, et al., Forced expression of terminal deoxynucleotidyl transferase in fetal thymus resulted in a decrease in gammadelta T cells and random dissemination of Vgamma3Vdelta1 T cells in skin of newborn but not adult mice. Immunology. 2000 Apr;99(4):489-97
• All: 2 reference(s)