Pkd1^tm1Hung
Targeted Allele Detail

Summary

• Symbol: Pkd1^tm1Hung
• Name: polycystin 1, transient receptor potential channel interacting; targeted mutation 1, Hung Li
• MGI ID: MGI:3612536
• Synonyms: Pkd1^L3
• Gene: Pkd1 Location: Chr17:24768808-24815482 bp, + strand Genetic Position: Chr17, 12.4 cM
• Alliance: Pkd1^tm1Hung page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104361
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An inverted mc1 promoter-driven neo flanked by two loxP sites was inserted into intron 34. A third loxP site was inserted into intron 30. RT-PCR detected a faint band. Wesetern blot detected a faint baind corresponding to amino acids 866-882. These analyses indicated that the insertion causes a significant disturbance but does not completely abrogate expresson of the gene. (J:104361)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pkd1 Mutation: 153 strains or lines available

References

• Original: J:104361 Jiang ST, et al., Defining a link with autosomal-dominant polycystic kidney disease in mice with congenitally low expression of pkd1. Am J Pathol. 2006 Jan;168(1):205-20
• All: 3 reference(s)