Jag1^tm1Vtlr
Targeted Allele Detail

Summary

• Symbol: Jag1^tm1Vtlr
• Name: jagged 1; targeted mutation 1, Verdon Taylor
• MGI ID: MGI:3612394
• Gene: Jag1 Location: Chr2:136923371-136958440 bp, - strand
• Alliance: Jag1^tm1Vtlr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:103728
• Parent Cell Line: GS1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A PGK-neo selection cassette flanked by two loxP sites was inserted upstream of exon 1 and a third loxP site was inserted into intron 2. Transient cre expression excised the neo. (J:103728)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Jag1 Mutation: 78 strains or lines available

Notes

Neural stem cells were isolated from postnatal Jag1^tmVtlr or Notch1^tm2Agt animals carrying the Cre-reporter Rosa26R allele. Inactivation of the floxed allele in dissociated cells was accomplished using an Adenoviral-Cre vector. The Jagged1- or Notch1-deficient cells were able to form, at similar rates, neurospheres that were indistinguishable in size and morphology from those formed by control cells. 5 days post-ablation, a 5-fold or 250-fold reduction in self-replicating stem cells was detected for Jagged1- or Notch1-deficient neurospheres. Differentiation potential was not affected for either Jagged1- or Notch1-deficient neural stem cells in culture. Similar results were found for neural stem cells isolated from the subventricular zone of adult mice carrying floxed Jagged1 or Notch1.

References

• Original: J:103728 Nyfeler Y, et al., Jagged1 signals in the postnatal subventricular zone are required for neural stem cell self-renewal. EMBO J. 2005 Oct 5;24(19):3504-15
• All: 6 reference(s)