Cbfa2t2^tm1Swh
Targeted Allele Detail

Summary

• Symbol: Cbfa2t2^tm1Swh
• Name: CBFA2/RUNX1 translocation partner 2; targeted mutation 1, Scott W Hiebert
• MGI ID: MGI:3607923
• Synonyms: Mtgr1^-
• Gene: Cbfa2t2 Location: Chr2:154278401-154381276 bp, + strand Genetic Position: Chr2, 76.62 cM, cytoband C3.2
• Alliance: Cbfa2t2^tm1Swh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:102172
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance gene was inserted into exon 7, which is within the first conserved domain that is found in all family members. RT-PCR analysis confirmed that a transcript lacking exon 7 was expressed from this allele, but both immunoblot analysis, and immunoprecipitation, followed by immunoblot analysis with purified antibodies directed to the N terminus of indicated that neither a full-length nor a truncated Mtgr1 protein was produced at detectable levels. (J:102172)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cbfa2t2^tm1Swh

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cbfa2t2 Mutation: 211 strains or lines available

References

• Original: J:102172 Amann JM, et al., Mtgr1 is a transcriptional corepressor that is required for maintenance of the secretory cell lineage in the small intestine. Mol Cell Biol. 2005 Nov;25(21):9576-85
• All: 4 reference(s)