Tg(Ins2-GAD2)1Lt
Transgene Detail

Summary

• Symbol: Tg(Ins2-GAD2)1Lt
• Name: transgene insertion 1, Edward H Leiter
• MGI ID: MGI:3606176
• Synonyms: RIP7-hGAD65, TgN(RIP7hGAD65)Lt4 Y-line
• Transgene: Tg(Ins2-GAD2)1Lt Location: unknown Genetic Position: ChrY, Syntenic
• Alliance: Tg(Ins2-GAD2)1Lt page

Transgene origin

• Strain of Origin: NOD/ShiLt

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-GAD2)1Lt expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GAD2 (2572)

  Gad2	(MGI:95634)

• Tg(Ins2-GAD2)1Lt expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: The transgene comprises the cDNA sequence encoding human glutamic acid decarboxylase 2 under control of a modified rat insulin 2 promoter composed of 9.5 kb of genomic DNA from upstream of the transcription initiation site fused to the first intron of Ins2. Transmission from the founder male only to male progeny demonstrated integration into the Y chromosome. Densitrometric Southern blot analysis indicates presence of approximately 17 copies of the transgene. Expression in the pancreatic islets of transgenic mRNA has been demonstrated by reverse transcription followed by PCR (RT-PCR), and of GAD2 protein by western blot analysis. (J:100251)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:100251 Bridgett M, et al., Differential protection in two transgenic lines of NOD/Lt mice hyperexpressing the autoantigen GAD65 in pancreatic beta-cells. Diabetes. 1998 Dec;47(12):1848-56
• All: 5 reference(s)