Insig1^tm1Mbjg
Targeted Allele Detail

Summary

• Symbol: Insig1^tm1Mbjg
• Name: insulin induced gene 1; targeted mutation 1, Joseph L Goldstein
• MGI ID: MGI:3603523
• Synonyms: Insig1^f, Insig1^tm1Jlg
• Gene: Insig1 Location: Chr5:28276410-28283660 bp, + strand Genetic Position: Chr5, 13.94 cM
• Alliance: Insig1^tm1Mbjg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101203
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A vector was designed to flank exon 1, containing the ATG, with loxP sites. An FRT and floxed neo was inserted upstream of the exon. Transcript was reduced by 20%, possibly due to transcriptional interference from the neo cassette. (J:101203)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Insig1 Mutation: 15 strains or lines available

References

• Original: J:101203 Engelking LJ, et al., Schoenheimer effect explained - feedback regulation of cholesterol synthesis in mice mediated by Insig proteins. J Clin Invest. 2005 Sep;115(9):2489-98
• All: 7 reference(s)