Gad1^tm1Tama
Targeted Allele Detail

Summary

• Symbol: Gad1^tm1Tama
• Name: glutamate decarboxylase 1; targeted mutation 1, Nobuaki Tamamaki
• MGI ID: MGI:3590300
• Synonyms: GAD67^GFP, GAD67-GFP
• Gene: Gad1 Location: Chr2:70391644-70432358 bp, + strand Genetic Position: Chr2, 41.63 cM, cytoband D
• Alliance: Gad1^tm1Tama page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86389
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An EGFP was inserted at the ATG in exon 1. A loxP-flanked neo was included in the vector. Western blot demonstrated that these mutants had less GFP in the cerebral cortex than when the neo was removed. (J:86389, J:144883)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gad1 Mutation: 64 strains or lines available

References

• Original: J:86389 Tamamaki N, et al., Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse. J Comp Neurol. 2003 Dec 1;467(1):60-79
• All: 48 reference(s)