Wap^tm1Kuw
Targeted Allele Detail

Summary

• Symbol: Wap^tm1Kuw
• Name: whey acidic protein; targeted mutation 1, Kay-Uwe Wagner
• MGI ID: MGI:3584430
• Gene: Wap Location: Chr11:6585482-6588637 bp, - strand Genetic Position: Chr11, 4.61 cM
• Alliance: Wap^tm1Kuw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:100003
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The first exon and the splicing signal at the first exon-intron junction have been replaced by a loxP-flanked Pgk-neo cassette. No whey acidic protein mRNA or protein is detectable by northern or western blot analysis, respectively, in the mammary glands or milk on day 1 of lactation of dams homozygous for the targeted allele, nor is the protein detected in their secretory alveoli by immunohistochemical analysis. Expression in heterozygous dams does not differ significantly from that in wild-type mothers. (J:100003)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Wap Mutation: 33 strains or lines available

References

• Original: J:100003 Triplett AA, et al., Expression of the whey acidic protein (Wap) is necessary for adequate nourishment of the offspring but not functional differentiation of mammary epithelial cells. Genesis. 2005 Aug 16;43(1):1-11
• All: 1 reference(s)