Bcl6b^tm1Dtf
Targeted Allele Detail

Summary

• Symbol: Bcl6b^tm1Dtf
• Name: B cell CLL/lymphoma 6, member B; targeted mutation 1, Douglas T Fearson
• MGI ID: MGI:3584139
• Synonyms: Bcl5b^-
• Gene: Bcl6b Location: Chr11:70114954-70120624 bp, - strand Genetic Position: Chr11, 42.99 cM, cytoband B4
• Alliance: Bcl6b^tm1Dtf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99818
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting construct was designed such that nucleotides 14-114 of exon 2, encoding codons 1-33, were replaced with a human CD2 reporter cDNA and a loxP-flanked neomycin resistance gene in reverse orientation. Three copies of the SV40 poly A signal were inserted into exon 3. The first downstream in-frame methionine was at codon 119, distal to the poxvirus and zinc finger domain. Northern blot of mutant lung samples revealed a truncated transcript of 0.53 kb, and that the inserted sequence altered the normal splicing of the transcript. (J:99818)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bcl6b Mutation: 14 strains or lines available

References

• Original: J:99818 Manders PM, et al., BCL6b mediates the enhanced magnitude of the secondary response of memory CD8+ T lymphocytes. Proc Natl Acad Sci U S A. 2005 May 24;102(21):7418-25
• All: 1 reference(s)