F11r^{Gt(KST235)Byg}
Gene trapped Allele Detail

Summary

• Symbol: F11r^{Gt(KST235)Byg}
• Name: F11 receptor; gene trap KST235, BayGenomics
• MGI ID: MGI:3583782
• Synonyms: F11r^{Gt(pGT1pfs)1Upn}, JAM-A^-, Jam-A^gt
• Gene: F11r Location: Chr1:171265129-171292161 bp, + strand Genetic Position: Chr1, 79.43 cM, cytoband H2
• Alliance: F11r^{Gt(KST235)Byg} page

Mutation origin

• Mutant Cell Line: KST235
• Germline Transmission: Earliest citation of germline transmission: J:127992
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpf
• Mutation details: A gene trap vector (pGT1TMpfs) insertion resulted in the fusion of the first Ig domain to TMbetageo, an artificial protein created from the transmembrane domain of rat Cd4 joined to the catalytically active domains of betagalactosidase and neomycin genes followed by an IRES and placental alkaline phosphatase reporter cassette. The betagalactosidase and neomycin genes are flanked by Frt sites. The absence of protein product in the endothelium of the aorta was confirmed by immunohistochemistry. (J:99570)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  KST235	CC144732 ; (MGI Seq Detail)	5' RACE	unknown
  * GRCm39; (strand of gene trap mutagenesis)

Expression

In Mice Carrying this Mutation:

9 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F11r Mutation: 28 strains or lines available

References

• Original: J:99570 Parris JJ, et al., JAM-A expression during embryonic development. Dev Dyn. 2005 Aug;233(4):1517-24
• All: 11 reference(s)