Smad9^tm1Mwst
Targeted Allele Detail

Summary

• Symbol: Smad9^tm1Mwst
• Name: SMAD family member 9; targeted mutation 1, Michael Weinstein
• MGI ID: MGI:3581766
• Synonyms: Smad8^3loxP
• Gene: Smad9 Location: Chr3:54663003-54708678 bp, + strand Genetic Position: Chr3, 25.62 cM, cytoband D
• Alliance: Smad9^tm1Mwst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:99098
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: A targeting construct was designed to flank exon 3 with loxP sites and insert a PGK-neo into intron 3 with a 3' loxP site. RT-PCR demonstrated a hybrid transcript in mutant mice, utilizing a splice site 5 bp upstream of the initiationg ATG. This aberrant splicing caused neo to be out of frame with the endogenous transcript, resulting in a premature stop codon, and thereby allowing synthesis of a truncated Smad9 protein lacking about three-fourths of the MH2 domain. Real time ePCR demonstrated that transcript is reduced in the adult mutant brain, and that this allele is a hypomorph. (J:99098)

Expression

In Mice Carrying this Mutation:	8 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smad9 Mutation: 30 strains or lines available

References

• Original: J:99098 Hester M, et al., Smad1 and Smad8 function similarly in mammalian central nervous system development. Mol Cell Biol. 2005 Jun;25(11):4683-92
• All: 1 reference(s)