Lpar3^tm1Jch
Targeted Allele Detail

Summary

• Symbol: Lpar3^tm1Jch
• Name: lysophosphatidic acid receptor 3; targeted mutation 1, Jerold Chun
• MGI ID: MGI:3579139
• Synonyms: LPA3^-
• Gene: Lpar3 Location: Chr3:145926718-145991941 bp, + strand Genetic Position: Chr3, 71.03 cM
• Alliance: Lpar3^tm1Jch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98434
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 5' UTR, start codon and first 26 amino acids in exon 2 was replaced with a neomycin resistance gene in reverse orientation. Two types of truncated transcript were detected in mutant testis or kidney of by RT-PCR, one having the undeleted region of exon 2 and full-length exon 3, and the other having exons 1 and 3, but not 2. Western blot failed to detect corresponding proteins for these transcripts, indicating that neither can be properly translated. (J:98434)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lpar3 Mutation: 13 strains or lines available

References

• Original: J:98434 Ye X, et al., LPA3-mediated lysophosphatidic acid signalling in embryo implantation and spacing. Nature. 2005 May 5;435(7038):104-8
• All: 15 reference(s)