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Sema3btm1.2Brd
Targeted Allele Detail
Summary
Symbol: Sema3btm1.2Brd
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B; targeted mutation 1.2, Allan Bradley
MGI ID: MGI:3576464
Synonyms: m2, Sema3BBrdm2
Gene: Sema3b  Location: Chr9:107474873-107486428 bp, - strand  Genetic Position: Chr9, 58.31 cM
Alliance: Sema3btm1.2Brd page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:97316
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprt1b-m2
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsExons 1-6, encoding the first 163 amino acids and ~40% of the SEMA domain, were flanked with loxP sites. An FRT-flanked puromycin resistance cassette was inserted into intron 6. ES cells were transfected with Cre to remove the floxed sequence containine exons 1-6. In situ hybridization of mutant embryos demonstrated lack of expression in rib primordial and regions of the head. RT-PCR showed transcripts from exons 7 and higher, though the transcripts do not have the full-length SEMA domain and therefore result in a functionally null protein. (J:97316)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Sema3b Mutation:  31 strains or lines available
References
Original:  J:97316 van der Weyden L, et al., Null and conditional semaphorin 3B alleles using a flexible puroDeltatk loxP/FRT vector. Genesis. 2005 Apr;41(4):171-8
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory