Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Andrew P McMahon
• MGI ID: MGI:3576373
• Synonyms: Gt(ROSA)26Sor^{tm1(smo/YFP)Amc}, R26-lsl-SmoM2, R26SmoM2, R26^SmoM2, Rosa^{SmoM2 c}, SmoM2, SmoM2^fl, SmoM2/Yfp, SmoM2-YFP^fl, Smo^OEX
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89445
• Parent Cell Line: AV3 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Smo (MGI:108075)

• Mutation details: A loxP flanked PGK-neo-stop cassette upstream of the Yellow Fluorescent Protein (YFP) and mouse gene (SMO/EYFP) was inserted into the locus. The mutant allele consists of a fusion product involving YFP and the constitutively active W539L point mutation of the mouse gene (SmoM2), which corresponds to the W535L mutation in the human sequence. Expression of the fusion gene is blocked by a loxP-flanked STOP fragment placed between the endogenous promoter and the fusion sequence. (J:89445)

Disease models

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:89445 Jeong J, et al., Hedgehog signaling in the neural crest cells regulates the patterning and growth of facial primordia. Genes Dev. 2004 Apr 15;18(8):937-51
• All: 172 reference(s)