Dmd^tm1Khan
Targeted Allele Detail

Summary

• Symbol: Dmd^tm1Khan
• Name: dystrophin, muscular dystrophy; targeted mutation 1, Kazunori Hanaoka
• MGI ID: MGI:3531483
• Gene: Dmd Location: ChrX:81992476-84249747 bp, + strand Genetic Position: ChrX, 38.38 cM, cytoband C
• Alliance: Dmd^tm1Khan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96210
• Parent Cell Line: TT2F (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 3' targeting vector containing a loxP site was inserted downstream of exon 79. Subsequently 5' targeting vector containing second loxP site was inserted to replace brain-type promoter and its specific exon 1. In resulting mutant animals, the entire region of the Dmd gene was flanked by two loxP sites, and full-length expression in the brain was specifically inactivated. RT-PCR and Western blot analyses showed full length dystrophin expression in the cerebrum was specifically inactivated, with no effect on expressions of other isoforms. (J:96210)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dmd Mutation: 153 strains or lines available

References

• Original: J:96210 Kudoh H, et al., A new model mouse for Duchenne muscular dystrophy produced by 2.4Mb deletion of dystrophin gene using Cre-loxP recombination system. Biochem Biophys Res Commun. 2005 Mar 11;328(2):507-16
• All: 1 reference(s)