Casq1^{Gt(OST82566)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Casq1^{Gt(OST82566)Lex}
• Name: calsequestrin 1; gene trap OST82566, Lexicon Genetics
• MGI ID: MGI:3530841
• Synonyms: Casq1^{Gt(neo)587Lex}, CS1^-
• Gene: Casq1 Location: Chr1:172037461-172047435 bp, - strand Genetic Position: Chr1, 79.54 cM
• Alliance: Casq1^{Gt(OST82566)Lex} page

Mutation origin

• Mutant Cell Line: OST82566
• Germline Transmission: Earliest citation of germline transmission: J:140828
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector
• Mutation details: The retroviral gene trap vector VICTR24 integrated between exons 3 and 4 of the gene locus. This vector contains a splice acceptor sequence followed by a neomycin selection cassette, and a BTK gene. Gene inactivation was confirmed by immunoblotting of limb muscle homogenates prepared from homozyogtes. Immunoblotting also confirmed that there was no compensatory increase in expression of the Casq2 gene. (J:140828)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST82566	CG519317 ; (MGI Seq Detail)	3' RACE	Chr1:172040910-172044462 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  10 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Casq1 Mutation: 27 strains or lines available

References

• Original: J:140828 Paolini C, et al., Reorganized stores and impaired calcium handling in skeletal muscle of mice lacking calsequestrin-1. J Physiol. 2007 Sep 1;583(Pt 2):767-84
• All: 13 reference(s)