Spi1^tm1Nutt
Targeted Allele Detail

Summary

• Symbol: Spi1^tm1Nutt
• Name: Spi-1 proto-oncogene; targeted mutation 1, Stephen L Nutt
• MGI ID: MGI:3528071
• Synonyms: PU.1^gfp, Sfpi1^fl-gfp
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^tm1Nutt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95651
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting construct was designed to flank exon 5 with loxP sites and insert an FRT-flanked sequence containing a splice acceptor site, stop codons in all three reading frames, and an IRES-gfp-neo. Transcription resulting in the bicistronic mRNA was confirmed by flow cytometry. (J:95651)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 30 strains or lines available

References

• Original: J:95651 Nutt SL, et al., Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors. J Exp Med. 2005 Jan 17;201(2):221-31
• All: 15 reference(s)