Tg(RAB27A-EGFP/Rab27a)84Seab
Transgene Detail

Summary

• Symbol: Tg(RAB27A-EGFP/Rab27a)84Seab
• Name: transgene insertion 84, Miguel C Seabra
• MGI ID: MGI:3527487
• Transgene: Tg(RAB27A-EGFP/Rab27a)84Seab Location: unknown
• Alliance: Tg(RAB27A-EGFP/Rab27a)84Seab page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/Ca)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(RAB27A-EGFP/Rab27a)84Seab expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Rab27a (MGI:1861441)

• Mutation details: This transgene contains a human RAB27A promoter, a fusion gene of EGFP and the mouse Rab27a gene followed by a polyadenlyation sequence. Fluorescence microscopy and immunoblot analysis showed expression of EGFP in lines 84 and 90 mimics the endogenous gene expression pattern. (J:95342)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Crosses of lines 84 and 90 with Rab27a mice rescue the ashen phenotype. This is a representative record representing Tg(RAB27A-EGFP/Rab27a)84Seab and Tg(RAB27A-EGFP/Rab27a)90Seab, both of which have identical expression patterns, and Tg(RAB27A-EGFP/Rab27a)82Seab which has an unspecified expression pattern.

References

• Original: J:95342 Tolmachova T, et al., A general role for Rab27a in secretory cells. Mol Biol Cell. 2004 Jan;15(1):332-44
• All: 3 reference(s)