Gja1^tm4Kwi
Targeted Allele Detail

Summary

• Symbol: Gja1^tm4Kwi
• Name: gap junction protein, alpha 1; targeted mutation 4, Klaus Willecke
• MGI ID: MGI:3525196
• Synonyms: Cx43K258stop
• Gene: Gja1 Location: Chr10:56253297-56266519 bp, + strand Genetic Position: Chr10, 28.64 cM
• Alliance: Gja1^tm4Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:95050
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: In ES cells previously targeted for deletion of the coding sequence, a vector was used to insert the Gja1 genomic intron-exon 2 boundry and to change codon 258 to a stop codon. Northern blot of heart, brain and liver demonstrated that mutants expressed no wild-type mRNA. (J:95050)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gja1 Mutation: 59 strains or lines available

References

• Original: J:95050 Maass K, et al., Defective epidermal barrier in neonatal mice lacking the C-terminal region of connexin43. Mol Biol Cell. 2004 Oct;15(10):4597-608
• All: 10 reference(s)