Tg(HK2-luc)231Uku
Transgene Detail

Summary

• Symbol: Tg(HK2-luc)231Uku
• Name: transgene insertion 231, University of Kuopio
• MGI ID: MGI:3522676
• Synonyms: Uku231
• Transgene: Tg(HK2-luc)231Uku Location: unknown
• Alliance: Tg(HK2-luc)231Uku page

Transgene origin

• Strain of Origin: (BALB/c x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: This transgenic line carries 8-10 copies of a construct comprising 254 base pairs of 5' flanking DNA and 106 base pairs of untranslated sequence from exon I of the human hexokinase II gene joined to the firefly luciferase coding sequence, which is followed by the SV40 small intron and polyadenylation signal. (J:93560, J:95065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

No distinction was made among the 5 transgenic lines - this and Tg(HK2-luc)229Uku, 230Uku, 232Uku and 234Uku - carrying from 1-2 to 45-50 copies of the same construct; the authors state there were no significant differences among them, whether or not results were normalized to copy number. It was noted that baseline luciferase expression varied inversely with transgene copy number. Baseline luciferase activity in heart, skeletal muscle and adipose tissue of 4 to 5-week old mice bearing these transgenes was not statistically greater than background.

References

• Original: J:93560 Pirinen E, et al., Analysis of the human hexokinase II promoter in vivo: lack of insulin response within 4.0 kb. Biochim Biophys Acta. 2004 Jan 20;1676(2):149-54
• All: 2 reference(s)