Nkx6-2^tm1Gow
Targeted Allele Detail

Summary

• Symbol: Nkx6-2^tm1Gow
• Name: NK6 homeobox 2; targeted mutation 1, Alexander Gow
• MGI ID: MGI:3522433
• Synonyms: Nkx6-2^-
• Gene: Nkx6-2 Location: Chr7:139159292-139162713 bp, - strand Genetic Position: Chr7, 84.57 cM
• Alliance: Nkx6-2^tm1Gow page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94807
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A fragment of sequence containing the ORF in exons 1 and 2 was replaced with a lacZ-PGK-neo cassette. Northern blot of mutant brains indicated a lack of Nkx6-2 transcript, however, two signals were observed that hybridized to a lacZ probe. X-gal staining demonstrated lacZ was expressed prenatally in neurons and postnatally in oligodendrocytes of mutant embryos. (J:94807)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx6-2 Mutation: 16 strains or lines available

References

• Original: J:94807 Southwood C, et al., CNS myelin paranodes require Nkx6-2 homeoprotein transcriptional activity for normal structure. J Neurosci. 2004 Dec 15;24(50):11215-25
• All: 2 reference(s)