Kcnn1^tm1.1Jpad
Targeted Allele Detail

Summary

• Symbol: Kcnn1^tm1.1Jpad
• Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1; targeted mutation 1.1, John P Adelman
• MGI ID: MGI:3522422
• Synonyms: SK1delta
• Gene: Kcnn1 Location: Chr8:71294693-71315902 bp, - strand Genetic Position: Chr8, 34.27 cM
• Alliance: Kcnn1^tm1.1Jpad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94941
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted 40 nucleotides 5' of the initiation methionine codon in exon 3 and a floxed neomycin resistance gene as well as an EGFP gene were inserted in the intron between exons 5 and 6. Crossing to cre-deleter mice led to the excision of the coding portion of exon 3, exon 4 and exon 5 as well as the neo resistance gene. RT-PCR and western blot analysis confirmed the absence of transcription and translation. The EGFP insert was non functional. (J:94941)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnn1 Mutation: 23 strains or lines available

References

• Original: J:94941 Bond CT, et al., Small conductance Ca2+-activated K+ channel knock-out mice reveal the identity of calcium-dependent afterhyperpolarization currents. J Neurosci. 2004 Jun 9;24(23):5301-6
• All: 2 reference(s)