Slc46a2^tm1Moki
Targeted Allele Detail

Summary

• Symbol: Slc46a2^tm1Moki
• Name: solute carrier family 46, member 2; targeted mutation 1, Moon Kim
• MGI ID: MGI:3521803
• Synonyms: TDLacZ, TSCOT^LacZ
• Gene: Slc46a2 Location: Chr4:59905899-59915056 bp, - strand Genetic Position: Chr4, 32.81 cM
• Alliance: Slc46a2^tm1Moki page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140121
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 4.2-kb targeting vector consisting of IRES-LacZ with a neo-selectable marker was placed in between two BamHI sites found in the first exon of the gene locus. This vector replaced coding sequence for transmembrane domains 5 through 12. Northern blotting confirmed that the LacZ coding sequence was in a fusion transcript with the 5' portion of the gene's coding sequence. LacZ expression was detected in the thymic cortex and occasionally in the thymic corticomedullary junction. The N-terminal portion of the endogenous gene product, including transmembrane spans 1?4 of the protein, still remained expressed on the cell surface, as detected by flow cytometry. (J:140121)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc46a2 Mutation: 22 strains or lines available

References

• Original: J:140121 Ahn S, et al., TSCOT+ thymic epithelial cell-mediated sensitive CD4 tolerance by direct presentation. PLoS Biol. 2008 Aug 5;6(8):e191
• All: 2 reference(s)