Uba7^tm1Dez
Targeted Allele Detail

Summary

• Symbol: Uba7^tm1Dez
• Name: ubiquitin-like modifier activating enzyme 7; targeted mutation 1, Dong-Er Zhang
• MGI ID: MGI:3521787
• Synonyms: Uba7^tm1Dzh, Ube1l^-, Ube1L^-
• Gene: Uba7 Location: Chr9:107852766-107861255 bp, + strand Genetic Position: Chr9, 59.07 cM
• Alliance: Uba7^tm1Dez page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144149
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exon 14 encodes the active site for this enzyme that is responsible for activating ISG15 conjugation activity. LoxP sites were inserted upstream of exon 14 and downstream of exon 18. A Frt- and loxP- flanked neomycin resistance cassette was also inserted downstream of exon 18. Correct targeting was confirmed by Southern blotting. Splenocytes and thymocytes from homozygotes that were treated with IFN-beta expressed a larger mRNA transcript than controls due to the neomycin insertion. Tissue extracts from homozygote mice failed to demonstrate conjugating activity between ISG15 and larger protein complexes (i.e. no ISGylation activity). (J:144149)

Tumor Data

List all tumor models in MMHCdb carrying Uba7^tm1Dez

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Uba7 Mutation: 35 strains or lines available

References

• Original: J:144149 Kim KI, et al., Ube1L and protein ISGylation are not essential for alpha/beta interferon signaling. Mol Cell Biol. 2006 Jan;26(2):472-9
• All: 12 reference(s)