Cst8^tm1Itl
Targeted Allele Detail

Summary

• Symbol: Cst8^tm1Itl
• Name: cystatin 8 (cystatin-related epididymal spermatogenic); targeted mutation 1, Ingenious Targeting Laboratory
• MGI ID: MGI:3521755
• Synonyms: CRES^-, Cst8^-, Cst8^tm1Cnw
• Gene: Cst8 Location: Chr2:148640759-148647516 bp, + strand Genetic Position: Chr2, 73.56 cM
• Alliance: Cst8^tm1Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126945
• Parent Cell Line: iTL1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Not Specified
• Mutation details: A neo cassette replaced 4 kb of the genomic fragment, including part of exon 1 (5' untranslated region [UTR]) and all of exon 2. Western blot analysis confirmed the absence of protein expression in tissue lysates from the testis and epididymis of homozygous mutant mice. (J:168157)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cst8 Mutation: 8 strains or lines available

References

• Original: J:126945 von Horsten HH, et al., Oligomerization and transglutaminase cross-linking of the cystatin CRES in the mouse epididymal lumen: potential mechanism of extracellular quality control. J Biol Chem. 2007 Nov 9;282(45):32912-23
• All: 4 reference(s)