Mgat1^tm2Jxm
Targeted Allele Detail

Summary

• Symbol: Mgat1^tm2Jxm
• Name: mannoside acetylglucosaminyltransferase 1; targeted mutation 2, Jamey Marth
• MGI ID: MGI:3514311
• Synonyms: Mgat1^F
• Gene: Mgat1 Location: Chr11:49135018-49153854 bp, + strand Genetic Position: Chr11, 29.27 cM
• Alliance: Mgat1^tm2Jxm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94770
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Homologous recombination was used to flank the single coding exon with loxP sites. (J:94770)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mgat1 Mutation: 17 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Premature Ovarian Failure J:125880, J:237228.

In J:185823, deletion of the floxed Mgat1^tm2Jxm alleles by Tg(Sycp1-cre)4Min occurred with 0% efficiency. In contrast, deletion of the floxed Mgat1^tm2Jxm alleles by Tg(Stra8-icre)1Reb occurred with 100% efficiency in all six litters from two heterozygous males, giving 62 pups with no transmission of the floxed allele.

References

• Original: J:94770 Ye Z, et al., N-glycan branching requirement in neuronal and postnatal viability. Glycobiology. 2004 Jun;14(6):547-58
• All: 18 reference(s)