Gls^tm1Sray
Targeted Allele Detail

Summary

• Symbol: Gls^tm1Sray
• Name: glutaminase; targeted mutation 1, Stephen Rayport
• MGI ID: MGI:3514104
• Synonyms: Gls-
• Gene: Gls Location: Chr1:52202607-52272391 bp, - strand Genetic Position: Chr1, 26.86 cM
• Alliance: Gls^tm1Sray page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:108409
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: Floxed stop cassette was inserted by homologous recombination in Exon 1 preceding the ATG. Crossing these mice with tissue-specific cre expressors allows for tissue-specific rescue. Immumohistochemistry, Western, and QRT-PCR confirmed the absence of translation and transcription. (J:108409)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gls Mutation: 42 strains or lines available

References

• Original: J:108409 Masson J, et al., Mice lacking brain/kidney phosphate-activated glutaminase have impaired glutamatergic synaptic transmission, altered breathing, disorganized goal-directed behavior and die shortly after birth. J Neurosci. 2006 Apr 26;26(17):4660-71
• All: 8 reference(s)