Cpb2^tm1Nok
Targeted Allele Detail

Summary

• Symbol: Cpb2^tm1Nok
• Name: carboxypeptidase B2; targeted mutation 1, Noriko Okada
• MGI ID: MGI:3510646
• Synonyms: proCPR^-
• Gene: Cpb2 Location: Chr14:75479727-75520995 bp, + strand Genetic Position: Chr14, 39.73 cM, cytoband D2
• Alliance: Cpb2^tm1Nok page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93733
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 4 and 5, encoding some of the zinc and substrate binding sites, were replaced with a neomycin resistance gene. RT-PCR of mutant liver samples confirmed successful recombination, with bands shorter than wild-type by 200 bp, the length of exons 4 and 5. proCPR^- (J:93733)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpb2 Mutation: 30 strains or lines available

References

• Original: J:93733 Asai S, et al., Absence of procarboxypeptidase R induces complement-mediated lethal inflammation in lipopolysaccharide-primed mice. J Immunol. 2004 Oct 1;173(7):4669-74
• All: 1 reference(s)