Depmq1^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Depmq1^C57BL/6J
• Name: dependence on morphine QTL 1; C57BL/6J
• MGI ID: MGI:3510110
• QTL: Depmq1 Location: unknown Genetic Position: Chr1, cM position of peak correlated region/allele: 44.69 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased morphine withdrawal jumping frequency compared to 129P3/J. (J:93071)
• Inheritance: Recessive

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:93071

Linkage analysis was performed on a population of (C57BL/6J x 129P3/J)F2 intercross animals to identify QTLs associated with morphine withdrawal. 87 polymorphic markers were genotyped in an initial panel of 124 F2 animals. An additional 100 F2 animals were genotyped to refine the candidate locus. Parental strain C57BL/6J exhibits 10-fold greater morphine withdrawal jumping frequency compared to parental strain 129P3/J. Animals were administered morphine injection three times a day over 4 days and withdrawalwas induced with nalaxone on day 5.

Significant linkage mapped to an interval on mouse Chromosome 1 spanning 32 cM - 60 cM. This locus is named Depmq1 (dependence on morphine QTL 1). Depq1 reaches peak linkage at 51 cM near D1Mit48 with LOD=4.7. C57BL/6J-derived alleles confer increased withdrawal jumping frequency with a recessive mode of inheritance. Depmq1 accounts for 20% of the phenotypic variance. Depmq1 maps near a previously identified QTL for locomotor activity named Hdis at 54 cM on mouse Chromosome 1. Potential candidate genes mapping near Depmq1 are Plcd4 (39.2 cM) and Ugt1a1 (51.7 cM).

Suggestive loci mapped to 24 cM (LOD=2.3) on mouse Chromosome 5 and 45 cM (LOD=2.9) on mouse Chromosome 10.

Depmq1 combined with the 2 suggestive QTLs account for 43% of the phenotypic variance. Pairwise scanning did not detect interaction among the 3 loci.

References

• Original: J:93071 Kest B, et al., Mapping of a quantitative trait locus for morphine withdrawal severity. Mamm Genome. 2004 Aug;15(8):610-7
• All: 1 reference(s)