Mcph1^{Tg(HLA-A2.1)1Enge}
Transgenic Allele Detail

Summary

• Symbol: Mcph1^{Tg(HLA-A2.1)1Enge}
• Name: microcephaly, primary autosomal recessive 1; transgene insertion 1, Victor H Engelhard
• MGI ID: MGI:3056375
• Gene: Mcph1 Location: Chr8:18645147-18853205 bp, + strand Genetic Position: Chr8, 10.24 cM, cytoband A3
• Alliance: Mcph1^{Tg(HLA-A2.1)1Enge} page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Duplication, Insertion
• Mutation details: The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. Line 1 inserted into the gene at 18736683-18757058 (Build GRCm38/mm10) resulting in a duplication. The duplication results in a functional knock-out. Founder line 1 has a copy number of 3-8. (J:52612)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 7 strains available Cell Lines: 0 lines available
• Carrying any Mcph1 Mutation: 58 strains or lines available

Notes

Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2.

References

• Original: J:52612 Le AX, et al., Cytotoxic T cell responses in HLA-A2.1 transgenic mice. Recognition of HLA alloantigens and utilization of HLA-A2.1 as a restriction element. J Immunol. 1989 Feb 15;142(4):1366-71
• All: 23 reference(s)