Adgrg2^tm1Bend
Targeted Allele Detail

Summary

• Symbol: Adgrg2^tm1Bend
• Name: adhesion G protein-coupled receptor G2; targeted mutation 1, Ben Davies
• MGI ID: MGI:3056121
• Synonyms: HE6^-
• Gene: Adgrg2 Location: ChrX:159173686-159281066 bp, + strand Genetic Position: ChrX, 73.95 cM
• Alliance: Adgrg2^tm1Bend page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93019
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 22 through 25 with a neomycin resistance gene, stop codons in all three reading frames, and an IRES-Beta-galactosidase cassette. The deleted sequence encoded the 7 transmembrane domain. Northern blot analysis of mutants indicated a lack of transcript for this domain. Two short, weakly expressed transcripts were present in mutants, one corresponding to exons 1-20 and the other corresponding to the truncated mRNA fused to Beta-gal. Ablation of wild-type protein was confirmed by Western blot of epididymal membrane samples, and lacZ activity was demonstrated by BLUO-GAL staining. (J:93019)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adgrg2 Mutation: 12 strains or lines available

References

• Original: J:93019 Davies B, et al., Targeted deletion of the epididymal receptor HE6 results in fluid dysregulation and male infertility. Mol Cell Biol. 2004 Oct;24(19):8642-8
• All: 2 reference(s)