Fosl1^tm2.1Wag
Targeted Allele Detail

Summary

• Symbol: Fosl1^tm2.1Wag
• Name: fos-like antigen 1; targeted mutation 2.1, Erwin F Wagner
• MGI ID: MGI:3055993
• Synonyms: fra-1^-
• Gene: Fosl1 Location: Chr19:5497726-5505966 bp, + strand Genetic Position: Chr19, 4.33 cM
• Alliance: Fosl1^tm2.1Wag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93053
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Crossing Fosl1^tm2Wag with mice expressing Cre in the embryo proper created this allele in which the floxed region containing exons 3 and 4 (containing the dimerization and the DNA-binding domains) was excised. Upon excision, the 3' GFP was spliced to the N-terminal part of the transcript and was thereby activated. Western blot detected the fusion protein using an anti-GFP antibody. Immunofluorescence confirmed GFP activation. (J:93053)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fosl1 Mutation: 15 strains or lines available

References

• Original: J:93053 Eferl R, et al., The Fos-related antigen Fra-1 is an activator of bone matrix formation. EMBO J. 2004 Jul 21;23(14):2789-99
• All: 5 reference(s)