Rho^{tm1(EDNRB)Fji}
Targeted Allele Detail

Summary

• Symbol: Rho^{tm1(EDNRB)Fji}
• Name: rhodopsin; targeted mutation 1, Yoshinori Fujiyoshi
• MGI ID: MGI:3055163
• Synonyms: hETBR-CT, Rho^{tm1(hETBR)Fji}
• Gene: Rho Location: Chr6:115908709-115916997 bp, + strand Genetic Position: Chr6, 53.72 cM
• Alliance: Rho^{tm1(EDNRB)Fji} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:98779
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 1 was replaced with cDNA encoding a human endothelial receptor type B fused to the C-terminal 10 amino acids of rhodopsin immediately before the stop codon and 0.9 kb of the 3' UTR sequence with three pA signals and a PGK-neo selection cassette. Northern blot of mutant demonstrated successful expression of the knock-in sequence under endogenous control sequences. Transcripts of three different lengths were detected, corresponding to location of the three pA signals. Quantitative RT-PCR detected levels of mutant transcripts were present at ~8.7% of wild-type levels. (J:98779)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rho Mutation: 53 strains or lines available

References

• Original: J:98779 Kodama T, et al., Expression and localization of an exogenous G protein-coupled receptor fused with the rhodopsin C-terminal sequence in the retinal rod cells of knockin mice. Exp Eye Res. 2005 Jun;80(6):859-69
• All: 1 reference(s)