Tg(CAG-EGFP)131Osb
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP)131Osb
• Name: transgene insertion 131, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:3055151
• Synonyms: Osb-Y01, Tg14(act-EGFP)ObsY01, Tg(ACTB-EGFP)131Osb, Tg(ACTB-EGFP)Y01Osb, Tg(Act-EGFP)C14-Y01-FM131Osb, Tg(act-EGFP)Y01Osb, Tg(CAG-EGFP)C14-Y01-FM131Osb, TgN(act-EGFP)ObsC14-Yo1-FM1310, Y01
• Transgene: Tg(CAG-EGFP)131Osb Location: unknown Genetic Position: Chr14, Syntenic
• Alliance: Tg(CAG-EGFP)131Osb page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene comprises the cDNA encoding enhanced green fluorescent protein (EGFP), from the jellyfish Aequoria victoria, downstream of the "CAG promoter" -- which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene -- and followed by the rabbit beta-hemoglobin polyadenylation signal and 3' flanking sequence. (J:87415)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 8 strains available Cell Lines: 0 lines available

Notes

This transgene was made using the same construct as Tg(ACTB-EGFP)1Osb, but in this case it was injected into C57BL/6 fertilized oocytes. According to the Center for Animal Resources and Development Database (CARD R-Base), which distributes it, the transgene integrated on Chr 14D1.

References

• Original: J:87415 Okabe M, et al., 'Green mice' as a source of ubiquitous green cells. FEBS Lett. 1997 May 5;407(3):313-9
• All: 221 reference(s)