Prkag3^tm1Sson
Targeted Allele Detail

Summary

• Symbol: Prkag3^tm1Sson
• Name: protein kinase, AMP-activated, gamma 3 non-catalytic subunit; targeted mutation 1, Leif Andersson
• MGI ID: MGI:3055131
• Synonyms: AMPKgamma3 KO, gamma3 AMPK KO, Prkag3^-
• Gene: Prkag3 Location: Chr1:74778081-74788380 bp, - strand Genetic Position: Chr1, 38.54 cM
• Alliance: Prkag3^tm1Sson page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92821
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The insertion of a neomycin resistance gene resulted in the transcription of exons 1-4 joined to exons 11-13, with a frame shift after residue 211 and a premature stop codon at residue 235, skipping most of the 489 amino acids encoded by the wild-type transcript. Western blot showed the complete absence of wild-type protein in skeletal muscle. (J:92821)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkag3 Mutation: 26 strains or lines available

References

• Original: J:92821 Barnes BR, et al., The 5'-AMP-activated protein kinase gamma3 isoform has a key role in carbohydrate and lipid metabolism in glycolytic skeletal muscle. J Biol Chem. 2004 Sep 10;279(37):38441-7
• All: 14 reference(s)