Tg(Nes-Hspa1b-EGFP)50Okn
Transgene Detail
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Symbol: |
Tg(Nes-Hspa1b-EGFP)50Okn |
Name: |
transgene insertion 50, Hideyuki Okano |
MGI ID: |
MGI:3053165 |
Synonyms: |
E/nestin:EGFP, E/nestin:EGFP (line 50) |
Transgene: |
Tg(Nes-Hspa1b-EGFP)50Okn Location: unknown
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Alliance: |
Tg(Nes-Hspa1b-EGFP)50Okn page
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Strain of Origin: |
Not Specified
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Transgene Type: |
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Transgenic (Reporter) |
Mutation: |
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Insertion
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Mutation details: Enhanced green fluorescent protein is expressed from the Hspa1b promoter under control of the second-intronic transcription enhancer of the rat nestin gene. Cells from embryonic brain that expressed high levels of EGFP included a subpopulation having characteristics of neural stem cells (NSCs) - capability for proliferation, self-renewal, and generation of both neural and glial cells - whereas few putative NSCs were observed among low- or non-expressing cells. In adult mice, EGFP expressing cells, which occur only in the periventricular region, include a population of slowly dividing, multipotential progenitor cells that likely represent adult stem cells.
(J:91456)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Of six transgenic lines generated, only that designated #25 is described in the reference; it was noted that all lines had similar expression patterns.
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Original: |
J:91456 Kawaguchi A, et al., Nestin-EGFP transgenic mice: visualization of the self-renewal and multipotency of CNS stem cells. Mol Cell Neurosci. 2001 Feb;17(2):259-73 |
All: |
3 reference(s) |
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