Tg(pPWL512hyg)1Ems
Transgene Detail

Summary

• Symbol: Tg(pPWL512hyg)1Ems
• Name: transgene insertion 1, Elizabeth M Simpson
• MGI ID: MGI:3052969
• Transgene: Tg(pPWL512hyg)1Ems Location: unknown
• Alliance: Tg(pPWL512hyg)1Ems page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• The transgene contains a PGK promoter and the bacterial hygromycin resistance gene. (J:92459)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Transgene also called "feeder" and "hyg."

Transgenic mice on a C57BL/6 background express the hygromycin resistance gene in a wide variety of tissues including muscle. Embryonic feeder layers derived from these mice are resistant to up to 0.8mg/ml hygromycin B, whereas feeder layers from normal C57BL/6J mice are killed by 0.2mg/ml hygromycin B.

Mouse embryonic fibroblasts prepared from homozygous transgenic mice that are also homozygous for Tg(Hprt1)1Jae and Dnmt1^tm3Jae are resistant to concentrations of the drugs G418, 6TG, puromycin, and hygromycin well above those used normally for the selection of drug-resistant ES cells.

References

• Original: J:92459 Johnson KA, et al., Transgenic mice for the preparation of hygromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selections. Nucleic Acids Res. 1995 Apr 11;23(7):1273-5
• All: 7 reference(s)