Myh10^tm3Rsad
Targeted Allele Detail

Summary

• Symbol: Myh10^tm3Rsad
• Name: myosin, heavy polypeptide 10, non-muscle; targeted mutation 3, Robert S Adelstein
• MGI ID: MGI:3052104
• Synonyms: B^CN, B^R709CN
• Gene: Myh10 Location: Chr11:68582385-68707458 bp, + strand Genetic Position: Chr11, 41.95 cM, cytoband B3
• Alliance: Myh10^tm3Rsad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92081
• Parent Cell Line: CMT-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Single point mutation
• Mutation details: An arginine to cysteine mutation at amino acid 709 was inserted in exon 17 by homologous recombination. A floxed neo was inserted into intron 15. Southern blot and PCR confirmed recombination. Western blot indicated that this is a hypomorphic allele: expression in mutant brains was reduced 73% from wild-type levels. (J:92081)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myh10 Mutation: 95 strains or lines available

References

• Original: J:92081 Ma X, et al., A point mutation in the motor domain of nonmuscle myosin II-B impairs migration of distinct groups of neurons. Mol Biol Cell. 2004 Jun;15(6):2568-79
• All: 8 reference(s)