Ubash3a^tm1Jni
Targeted Allele Detail

Summary

• Symbol: Ubash3a^tm1Jni
• Name: ubiquitin associated and SH3 domain containing, A; targeted mutation 1, James N Ihle
• MGI ID: MGI:3051962
• Synonyms: Sts2^-, Tula^-
• Gene: Ubash3a Location: Chr17:31426847-31465866 bp, + strand Genetic Position: Chr17, 15.84 cM
• Alliance: Ubash3a^tm1Jni page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89761
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An 11 kb fragment of sequence containing exons 6, 7 and part of 8 was replaced with a neomycin resistance gene. Southern blot confirmed correct targeting and Northern blot demonstrated an absence of mRNA in homozygotes. Western blot indicated absence of protein in mutants. (J:89761)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ubash3a Mutation: 35 strains or lines available

References

• Original: J:89761 Carpino N, et al., Regulation of ZAP-70 activation and TCR signaling by two related proteins, Sts-1 and Sts-2. Immunity. 2004 Jan;20(1):37-46
• All: 13 reference(s)