Rabgef1^tm2Glli
Targeted Allele Detail

Summary

• Symbol: Rabgef1^tm2Glli
• Name: RAB guanine nucleotide exchange factor (GEF) 1; targeted mutation 2, Stephen J Galli
• MGI ID: MGI:3051238
• Synonyms: Rabgef1^-
• Gene: Rabgef1 Location: Chr5:130200644-130243178 bp, + strand Genetic Position: Chr5, 68.78 cM, cytoband F
• Alliance: Rabgef1^tm2Glli page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91790
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: WBB6F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: Exon 2 was replaced with an IRES and floxed PGK-neo cassette. Southern blot confirmed recombination and RT-PCR indicated no mRNA in mutant pups. Immunoblot demonstrated lack of expression of the full-length protein in fetal liver mast cells. (J:91790)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rabgef1 Mutation: 67 strains or lines available

References

• Original: J:91790 Tam SY, et al., RabGEF1 is a negative regulator of mast cell activation and skin inflammation. Nat Immunol. 2004 Aug;5(8):844-52
• All: 1 reference(s)