Pparg^tm1Yec
Targeted Allele Detail

Summary

• Symbol: Pparg^tm1Yec
• Name: peroxisome proliferator activated receptor gamma; targeted mutation 1, Yuqing E Chen
• MGI ID: MGI:3050813
• Synonyms: Ppargamma2^-
• Gene: Pparg Location: Chr6:115337912-115467360 bp, + strand Genetic Position: Chr6, 53.41 cM, cytoband E3-F1
• Alliance: Pparg^tm1Yec page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91479
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The initiation codon and part of exon B of the Pparg2 isoform were replaced with a red fluorescence protein (RFP) gene and PGK-neo. RT-PCR confirmed lack of mRNA in mutants and Western blot shows that protein production was ablated. RFP was expressed from the Pparg2 promoter. (J:91479)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pparg Mutation: 42 strains or lines available

References

• Original: J:91479 Zhang J, et al., Selective disruption of PPARgamma 2 impairs the development of adipose tissue and insulin sensitivity. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10703-8
• All: 2 reference(s)