Nfat5^tm1Snh
Targeted Allele Detail

Summary

• Symbol: Nfat5^tm1Snh
• Name: nuclear factor of activated T cells 5; targeted mutation 1, Steffan N Ho
• MGI ID: MGI:3050805
• Synonyms: Nfat5^delta
• Gene: Nfat5 Location: Chr8:108020102-108106149 bp, + strand Genetic Position: Chr8, 53.93 cM, cytoband D2
• Alliance: Nfat5^tm1Snh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91482
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed neo was inserted in intron 5 and a loxP site in intron 7. Transient Cre expression deleted the larger floxed region, including exons 6 and 7. These exons encode amino acids 254-380, which comprise the N-terminal portion of the rel DNA-binding domain (DBD). Germ line transmission was verified by PCR and Southern blot. RNA and protein expression in mutant MEFs showed that the allele encodes a truncated protein containing an internal deletion as expected. Immunoreactivity was eliminated as demonstrated by using antisera against the DBD. (J:91482)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfat5 Mutation: 68 strains or lines available

References

• Original: J:91482 Go WY, et al., NFAT5/TonEBP mutant mice define osmotic stress as a critical feature of the lymphoid microenvironment. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10673-8
• All: 19 reference(s)