Slc4a2tm1Ges
Targeted Allele Detail
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Symbol: |
Slc4a2tm1Ges |
Name: |
solute carrier family 4 (anion exchanger), member 2; targeted mutation 1, Gary E Shull |
MGI ID: |
MGI:3050237 |
Synonyms: |
AE2- |
Gene: |
Slc4a2 Location: Chr5:24628939-24645945 bp, + strand Genetic Position: Chr5, 11.74 cM
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Alliance: |
Slc4a2tm1Ges page
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Growth retardation and lack of incisor tooth eruption in Slc4a2tm1Ges/Slc4a2tm1Ges mice
Show the 6 phenotype image(s) involving this allele.
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Germline Transmission: |
Earliest citation of germline transmission:
J:91617
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A neo cassette replaced exons 14-17. Transcripts were expected to utilize the neo polyA, thereby lacking codons 703-1237, which encode the transmembrane domains. Northern blot using probes of sequences 3' to the neo showed that wild-type mRNAs were ablated in mutants, whereas probes of sequences 5' to the neo indicated presence of transcripts consistent in size with two splice variants. An immunofluorescence assay using an antibody that recognizes the C terminus confirmed protein disruption.
(J:91617)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:91617 Gawenis LR, et al., Mice with a targeted disruption of the AE2 Cl-/HCO3- exchanger are achlorhydric. J Biol Chem. 2004 Jul 16;279(29):30531-9 |
All: |
10 reference(s) |
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