Ptk7^{Gt(Betageo)1Matl}
Gene trapped Allele Detail

Summary

• Symbol: Ptk7^{Gt(Betageo)1Matl}
• Name: PTK7 protein tyrosine kinase 7; gene trap 1, Marc Tessier-Lavigne
• MGI ID: MGI:3046905
• Synonyms: Ptk7^XST87, XST87
• Gene: Ptk7 Location: Chr17:46875397-46940430 bp, - strand Genetic Position: Chr17, 22.9 cM, cytoband B3
• Alliance: Ptk7^{Gt(Betageo)1Matl} page

Mutation origin

• Mutant Cell Line: XST087
• Germline Transmission: Earliest citation of germline transmission: J:91298
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1TMpfs Vector Type: secretory trap
• Mutation details: A gene trap vector including a beta-galactosidase and neo was inserted into intron 2. The allele is predicted to result in a null or a severely hypomorphic mutation, with the in-frame fusion of the first 114 amino acids to the transmembrane Beta-gal and neo construct. A transcript with the expected size for the fused RNA was detected in Northern blot of mutant brain lysates, and Western blot showed a peptide of the size of the predicted fusion product instead of the two peptides detected in wild-type brain lysates. (J:91298)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XST087	CC156422 ; (MGI Seq Detail)	5' RACE	Chr17:46902440-46902702 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  37 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	2 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptk7 Mutation: 57 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:91298 Lu X, et al., PTK7/CCK-4 is a novel regulator of planar cell polarity in vertebrates. Nature. 2004 Jul 1;430(6995):93-8
• All: 9 reference(s)